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验证数据展示

  • WB analysis of HEK-293 using 16639-1-AP

    WB analysis of HEK-293 using 16639-1-AP

    WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells.

  • IHC staining of human prostate cancer using 16639-1-AP

    IHC staining of human prostate cancer using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IP experiment of mouse liver using 16639-1-AP

    IP experiment of mouse liver using 16639-1-AP

    IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • IP experiment of HepG2 using 16639-1-AP

    IP experiment of HepG2 using 16639-1-AP

    IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:8000) with HepG2 cells lysate 1480 ug.

  • IF Staining of PC-3 using 16639-1-AP

    IF Staining of PC-3 using 16639-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HUVEC using 16639-1-AP

    WB analysis of HUVEC using 16639-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 16639-1-AP

    IHC staining of mouse stomach using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of human pancreas cancer using 16639-1-AP

    IHC staining of human pancreas cancer using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of human placenta using 16639-1-AP

    IHC staining of human placenta using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of mouse kidney using 16639-1-AP

    IHC staining of mouse kidney using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of mouse pancreas using 16639-1-AP

    IHC staining of mouse pancreas using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of rat kidney using 16639-1-AP

    IHC staining of rat kidney using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of rat pancreas using 16639-1-AP

    IHC staining of rat pancreas using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of rat stomach using 16639-1-AP

    IHC staining of rat stomach using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IHC staining of human placenta using 16639-1-AP

    IHC staining of human placenta using 16639-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPLIN antibody included
  • IF Staining of HepG2 using 16639-1-AP

    IF Staining of HepG2 using 16639-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

EPLIN Polyclonal antibody

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16639-1-AP

产品说明书

引用文献(9)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

LIMA1, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624, SREBP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, HEK-293 cells, human placenta tissue
Positive IP detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue, rat pancreas tissue, mouse pancreas tissue, mouse kidney tissue, rat kidney tissue, rat stomach tissue, mouse stomach tissue, human placenta tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® EPLIN Ready-To-Use IHC Kit. EPLIN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16639-1-AP targets EPLIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag9994

Product name: Recombinant human LIMA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 261-401 aa of BC001247

Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain and actin binding 1
别名 LIMA1, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624, SREBP3
计算分子量 85 kDa
观测分子量 90 kDa, 110 kDa
GenBank蛋白编号BC001247
基因名称 EPLIN
Gene ID (NCBI) 51474
RRIDAB_2136657
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHB6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. The 110 kDa EPLIN-β isoform represents the full-length protein and the 90 kDa EPLIN-α isoform lacks the amino-terminal 160 amino acids. EPLIN-a expression is often down-regulated in cancerous cells and tissues. (PMID: 10806352, 31644899)

实验方案

Product Specific Protocols
IF protocol for EPLIN antibody 16639-1-APDownload protocol
IHC protocol for EPLIN antibody 16639-1-APDownload protocol
IP protocol for EPLIN antibody 16639-1-APDownload protocol
WB protocol for EPLIN antibody 16639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Mol Med

The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Authors - Qianqian Yang
View Article
humanIHC

J Cell Biol

Rab40-Cullin5 complex regulates EPLIN and actin cytoskeleton dynamics during cell migration.

Authors - Erik S Linklater
View Article
humanIHC,IF

Cell Biol Toxicol

M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway

Authors - Chao Zhang
View Article
mouseWB

Int J Mol Sci

Inhibition of Breast Cancer Bone Metastasis by LRP5-Overexpressing Osteocytes via the LIMA1/MYO5B Signaling Axis.

Authors - Yaning Chen
  • KD Validated
View Article
humanWB

Front Cell Infect Microbiol

Screening and identification of protein 29 of Echinococcus granulosus interacting molecules

Authors - Zailing Shang
View Article
humanWB

Proteomics

A proteomic signature and potential pharmacological opportunities in the adaptive resistance to MEK and PI3K kinase inhibition in pancreatic cancer cells

Authors - Alain Aguilar-Valdés
View Article
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