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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67840-1-Ig (same clone as 67840-1-PBS)

    WB analysis using 67840-1-Ig (same clone as 67840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67840-1-Ig (LIG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67840-1-PBS in a different storage buffer formulation.

LIG1 Monoclonal antibody, PBS Only

LIG1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67840-1-PBS

产品说明书

CloneNo. 3D9G8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, Indirect ELISA

别名

DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LIG1 Ready-To-Use IHC Kit. LIG1 primary antibody included.

产品信息

67840-1-PBS targets LIG1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag12489

Product name: Recombinant human LIG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 670-919 aa of BC108318

Sequence: LVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ligase I, DNA, ATP-dependent
别名 DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent
计算分子量 919 aa, 102 kDa
观测分子量130 kDa
GenBank蛋白编号BC108318
基因名称 LIG1
Gene ID (NCBI) 3978
RRIDAB_2918602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18858
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.