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验证数据展示

  • WB analysis using 67840-1-Ig

    WB analysis using 67840-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67840-1-Ig (LIG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

LIG1 Monoclonal antibody

LIG1 Monoclonal Antibody for WB, ELISA
Cat No. 67840-1-Ig

产品说明书

CloneNo. 3D9G8
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat and More (1)

应用

WB, ELISA and More (1)

别名

DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy LIG1 Ready-To-Use IHC Kit. LIG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67840-1-Ig targets LIG1 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag12489

Product name: Recombinant human LIG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 670-919 aa of BC108318

Sequence: LVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ligase I, DNA, ATP-dependent
别名 DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent
计算分子量 919 aa, 102 kDa
观测分子量130 kDa
GenBank蛋白编号BC108318
基因名称 LIG1
Gene ID (NCBI) 3978
RRIDAB_2918602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18858
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

实验方案

Product Specific Protocols
WB protocol for LIG1 antibody 67840-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

bioRxiv

Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy

Authors - Zhihua Kang
View Article