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验证数据展示

  • WB analysis of mouse ovary using 26424-1-AP

    WB analysis of mouse ovary using 26424-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26424-1-AP (LHCGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26424-1-AP

    IHC staining of human thyroid cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 26424-1-AP

    IHC staining of human thyroid cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 26424-1-AP

    IHC staining of human ovary cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 26424-1-AP (LHCGR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse ovary using 26424-1-AP

    IF Staining of mouse ovary using 26424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (26424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26424-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA and More (1)

别名

LCGR, LGR2, LH R, LH receptor, LH/CG-R

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive WB detected inmouse ovary tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tissue, human thyroid cancer tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse ovary tissue
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26424-1-AP targets LHCGR in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag19259

Product name: Recombinant human LHCGR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 49-219 aa of BC157028

Sequence: AGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LHCGR
别名 LCGR, LGR2, LH R, LH receptor, LH/CG-R
计算分子量 699 aa, 79 kDa
观测分子量 100 kDa
GenBank蛋白编号BC157028
基因名称 LHCGR
Gene ID (NCBI) 3973
RRIDAB_2880511
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22888
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

实验方案

Product Specific Protocols
IF protocol for LHCGR antibody 26424-1-APDownload protocol
IHC protocol for LHCGR antibody 26424-1-APDownload protocol
WB protocol for LHCGR antibody 26424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Biol Reprod

Interferon-tau infusion into the ovine corpus luteum delays luteolysis

Authors - Aydin Guzeloglu
View Article
mouseWB,IF

Biochem Biophys Res Commun

Effects of hCG on DA neuronal death of Parkinson's disease

Authors - Shi-Min Wang
  • KD Validated
View Article
mouseIF

Biol Res

Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb

Authors - Nengliang Duan
View Article
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