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  • Featured Product
  • KD/KO Validated

LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26424-1-AP

Print datasheet

Synonyms

LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor

验证数据展示

  • WB analysis of mouse ovary using 26424-1-AP

    WB analysis of mouse ovary using 26424-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26424-1-AP (LHCGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26424-1-AP

    IHC staining of human thyroid cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 26424-1-AP

    IHC staining of human thyroid cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse ovary using 26424-1-AP

    IF Staining of mouse ovary using 26424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (26424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Jurkat using 26424-1-AP

    FC experiment of Jurkat using 26424-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LHCGR (26424-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse ovary tissue
Positive IHC detected inhuman thyroid cancer tissue, human ovary tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse ovary tissue
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26424-1-AP targets LHCGR in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen LHCGR fusion protein Ag19259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LHCGR
Synonyms LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor
Calculated Molecular Weight 699 aa, 79 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC157028
Gene Symbol LHCGR
Gene ID (NCBI) 3973
RRIDAB_2880511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

实验方案

Product Specific Protocols
WB protocol for LHCGR antibody 26424-1-APDownload protocol
IHC protocol for LHCGR antibody 26424-1-APDownload protocol
IF protocol for LHCGR antibody 26424-1-APDownload protocol
FC protocol for LHCGR antibody 26424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Biochem Biophys Res Commun

Effects of hCG on DA neuronal death of Parkinson's disease

Authors - Shi-Min Wang
  • KD Validated
View Article