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- KD/KO Validated
LGR6 Polyclonal antibody
LGR6 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, mouse testis tissue, human brain tissue |
| Positive IHC detected in | human testis tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17658-1-AP targets LGR6 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IHC, ELISA |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | LGR6 fusion protein Ag11909 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | leucine-rich repeat-containing G protein-coupled receptor 6 |
| Synonyms | GPCR, LGR6, VTS20631 |
| Calculated Molecular Weight | 915 aa, 99 kDa |
| Observed Molecular Weight | 104 kDa |
| GenBank Accession Number | BC047905 |
| Gene Symbol | LGR6 |
| Gene ID (NCBI) | 59352 |
| RRID | AB_2135163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBX8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LGR6 antibody 17658-1-AP | Download protocol |
| IHC protocol for LGR6 antibody 17658-1-AP | Download protocol |
| FC protocol for LGR6 antibody 17658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.
| ||
Mol Ther Oncolytics Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer. | ||
Proc Natl Acad Sci U S A The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation | ||
Aging (Albany NY) Comprehensive analysis of cuproptosis-related immune biomarker signature to enhance prognostic accuracy in gastric cancer |
