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Galectin-4 Polyclonal antibody

Galectin-4 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13391-1-AP

Print datasheet

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

验证数据展示

  • WB analysis using 13391-1-AP

    WB analysis using 13391-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13391-1-AP

    IHC staining of human breast cancer using 13391-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human breast cancer using 13391-1-AP

    IHC staining of human breast cancer using 13391-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IF Staining of COLO 320 using 13391-1-AP

    IF Staining of COLO 320 using 13391-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using Galectin-4 antibody (13391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 13391-1-AP

    FC experiment of MCF-7 using 13391-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug Galectin-4 antibody (13391-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inHT-29 cells, COLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13391-1-AP targets Galectin-4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Galectin-4 fusion protein Ag4049 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 4
Synonyms Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4
Calculated Molecular Weight 323 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC034750
Gene Symbol LGALS4
Gene ID (NCBI) 3960
RRIDAB_2137072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56470
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

实验方案

Product Specific Protocols
WB protocol for Galectin-4 antibody 13391-1-APDownload protocol
IHC protocol for Galectin-4 antibody 13391-1-APDownload protocol
IF protocol for Galectin-4 antibody 13391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols