验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
32817-1-AP targets LEPR in WB, IF, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Eg2930 Product name: Recombinant Mouse Lepr protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-839 aa of NM_146146.2 Sequence: LNLAYPISPWKFKLFCGPPNTTDDSFLSPAGAPNNASALKGASEAIVEAKFNSSGIYVPELSKTVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLFICHMEPLPKNPFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVPRAKLNYALLMYLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDGNLKISWDSQTMAPFPLQYQVKYLENSTIVREAAEIVSATSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHCIYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRYAELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQRDGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTGLLKVSWEKPVFPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTLVMDVKVPMRGPEFWRKMDGDVTKKERNVTLLWKPLTKNDSLCSVRRYVVKHRTAHNGTWSEDVGNRTNLTFLWTEPAHTVTVLAVNSLGASLVNFNLTFSWPMSKVSAVESLSAYPLSSSCVILSWTLSPDDYSLLYLVIEWKILNEDDGMKWLRIPSNVKKFYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leptin receptor |
| 别名 | B219, CD295, Db, LEP-R, Leptin receptor |
| 计算分子量 | 131 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | NM_146146.2 |
| 基因名称 | Lepr |
| Gene ID (NCBI) | 16847 |
| RRID | AB_3742753 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P48356-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Leptin receptor (LEPR) is a protein-coding gene that encodes a receptor for leptin, an adipocyte-specific hormone that regulates body weight and fat metabolism (PMID: 11861497; 16333525). LEPR is involved in various physiological processes, including energy homeostasis, immune function, and hematopoiesis (PMID: 11923481; 25383904). It activates the JAK2/STAT3 signaling pathway, which is crucial for mediating leptin's effects on gene transcription and cellular responses (PMID: 11923481). LEPR also plays a role in the regulation of lipid metabolism, blood pressure, and blood glucose levels (PMID: 29254602).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LEPR antibody 32817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
