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验证数据展示

  • WB analysis of A2780 using 13336-1-AP (same clone as 13336-1-PBS)

    WB analysis of A2780 using 13336-1-AP (same clone as 13336-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 13336-1-AP (LENG8 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13336-1-PBS in a different storage buffer formulation.

  • WB analysis using 13336-1-AP (same clone as 13336-1-PBS)

    WB analysis using 13336-1-AP (same clone as 13336-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13336-1-AP (LENG8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13336-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 13336-1-AP (same clone as 13336-1-PBS)

    FC experiment of HEK-293 using 13336-1-AP (same clone as 13336-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug LENG8 Polyclonal antibody (13336-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13336-1-PBS in a different storage buffer formulation.

LENG8 Polyclonal antibody, PBS Only

LENG8 Polyclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 13336-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

KIAA1932, pp13842

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13336-1-PBS targets LENG8 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4153

Product name: Recombinant human LENG8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 501-800 aa of BC028048

Sequence: EDPERELKKQKRAARFQHGHSRRLRLEPLVLQMSSLESSGADPDWQELQIVGTCPDITKHYLRLTCAPDPSTVRPVAVLKKSLCMVKCHWKEKQDYAFACEQMKSIRQDLTVQGIRTEFTVEVYETHARIALEKGDHEEFNQCQTQLKSLYAENLPGNVGEFTAYRILYYIFTKNSGDITTELAYLTRELKADPCVAHALALRTAWALGNYHRFFRLYCHAPCMSGYLVDKFADRERKVALKAMIKTFRPALPVSYLQAELAFEGEAACRAFLEPLGLAYTGPDNSSIDCRLSLAQLSAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leukocyte receptor cluster (LRC) member 8
别名 KIAA1932, pp13842
计算分子量 800 aa, 88 kDa
观测分子量86-88 kDa
GenBank蛋白编号BC028048
基因名称 LENG8
Gene ID (NCBI) 114823
RRIDAB_1167573
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PV6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LENG8 is the core factor of the key RNA quality control system discovered in recent years, which plays a goalkeeper role in the regulation of eukaryotic gene expression. LENG8 promotes the recruitment of PAXT complex and RNA exonuclease, and mediates the degradation of abnormal RNA. It is a multifunctional evolutionarily conserved protein, which plays a key role from yeast to human beings. It is an important node molecule connecting gene post-transcriptional processing, RNA quality control and cell energy metabolism.

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