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LENG8 Polyclonal antibody

LENG8 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13336-1-AP

Print datasheet

Synonyms

KIAA1932, LENG8, pp13842

验证数据展示

  • WB analysis of A2780 using 13336-1-AP

    WB analysis of A2780 using 13336-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 13336-1-AP (LENG8 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 13336-1-AP

    FC experiment of HEK-293 using 13336-1-AP

    1X10^6 HEK-293 cells were stained with 0.2ug LENG8 antibody (13336-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • WB analysis using 13336-1-AP

    WB analysis using 13336-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13336-1-AP (LENG8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA2780 cells, Jurkat cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13336-1-AP targets LENG8 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LENG8 fusion protein Ag4153 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leukocyte receptor cluster (LRC) member 8
Synonyms KIAA1932, LENG8, pp13842
Calculated Molecular Weight 800 aa, 88 kDa
Observed Molecular Weight86-88 kDa
GenBank Accession NumberBC028048
Gene Symbol LENG8
Gene ID (NCBI) 114823
RRIDAB_1167573
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LENG8 antibody 13336-1-APDownload protocol
FC protocol for LENG8 antibody 13336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye
View Article