验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue, mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83586-1-RR targets LEFTY1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag4925 Product name: Recombinant human LEFTY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-366 aa of BC027883 Sequence: EQLLGSLLRQLQLKEVPTLDRADMEELVIPTHVRAQYVALLQRSHGDRSRGKRFSQSFREVAGRFLALEASTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSARARVTVEWLRVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPLASGAHKLVRFASQGAPAGLGEPQLELHTLDLGDYGAQGDCDPEAPMTEGTRCCRQEMYIDLQGMKWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASDGALVPRRLQP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | left-right determination factor 1 |
| 别名 | LEFTB, LEFTY1, LEFTYB, Protein lefty 1, Protein lefty B |
| 计算分子量 | 366 aa, 41 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC027883 |
| 基因名称 | LEFTY1 |
| Gene ID (NCBI) | 10637 |
| RRID | AB_3671200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O75610 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lefty is a member of the transforming growth factor (TGF) β superfamily, which is implicated in left-right patterning during embryogenesis. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LEFTY1 antibody 83586-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
