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验证数据展示

  • WB analysis of mouse skeletal muscle using 11004-1-AP

    WB analysis of mouse skeletal muscle using 11004-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 11004-1-AP

    IHC staining of mouse heart using 11004-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11004-1-AP (LDB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LDB3 Polyclonal antibody

LDB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11004-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11004-1-AP targets LDB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 LDB3 fusion protein Ag1417 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain binding 3
别名 CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP
计算分子量 32/78 kDa
观测分子量 32 kDa, 78-80 kDa
GenBank蛋白编号BC010929
基因名称 LDB3
Gene ID (NCBI) 11155
RRIDAB_2137049
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LDB3 antibody 11004-1-APDownload protocol
IHC protocol for LDB3 antibody 11004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

α-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilisation.

Authors - Garton F F
View Article
mouse, humanWB

Hum Mol Genet

Analysis of the ACTN3 Heterozygous Genotype Suggests That α-Actinin-3 Controls Sarcomeric Composition and Muscle Function in a Dose-Dependent Fashion.

Authors - Marshall W Hogarth
View Article
humanIHC

NPJ Precis Oncol

Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling

Authors - Shinji Kohsaka
View Article