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LDB3 Polyclonal antibody

LDB3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 11004-1-AP

Print datasheet

Synonyms

CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP

验证数据展示

  • WB analysis of mouse skeletal muscle using 11004-1-AP

    WB analysis of mouse skeletal muscle using 11004-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11004-1-AP (LDB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 11004-1-AP

    IHC staining of mouse heart using 11004-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11004-1-AP (LDB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11004-1-AP targets LDB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen LDB3 fusion protein Ag1417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM domain binding 3
Synonyms CYPHER, KIAA01613, KIAA0613, LDB3, ldb3z1, ldb3z4, LIM domain binding 3, LIM domain binding protein 3, ORACLE, PDLIM6, Protein cypher, ZASP
Calculated Molecular Weight 32/78 kDa
Observed Molecular Weight 32 kDa, 78-80 kDa
GenBank Accession NumberBC010929
Gene Symbol LDB3
Gene ID (NCBI) 11155
RRIDAB_2137049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LDB3 antibody 11004-1-APDownload protocol
IHC protocol for LDB3 antibody 11004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Hum Mol Genet

α-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilisation.

Authors - Garton F F
View Article
mouse, humanWB

Hum Mol Genet

Analysis of the ACTN3 Heterozygous Genotype Suggests That α-Actinin-3 Controls Sarcomeric Composition and Muscle Function in a Dose-Dependent Fashion.

Authors - Marshall W Hogarth
View Article
humanIHC

NPJ Precis Oncol

Identification of novel prognostic and predictive biomarkers in salivary duct carcinoma via comprehensive molecular profiling

Authors - Shinji Kohsaka
View Article