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验证数据展示

  • WB analysis of HeLa using 14476-1-AP

    WB analysis of HeLa using 14476-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14476-1-AP (LCOR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 14476-1-AP

    IHC staining of human endometrial cancer using 14476-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human endometrial cancer using 14476-1-AP

    IHC staining of human endometrial cancer using 14476-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14476-1-AP

    IF Staining of HeLa using 14476-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14476-1-AP (LCOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

LCOR Polyclonal antibody

LCOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14476-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MLR2, Mblk1-related protein 2, Ligand-dependent corepressor, C10orf12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14476-1-AP targets LCOR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5851

Product name: Recombinant human LCOR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-406 aa of BC053359

Sequence: MQRMIQQFAAEYTSKNSSTQDPSQPNSTKNQSLPKASPVTTSPTAATTQNPVLSKLLMADQDSPLDLTVRKSQSEPSEQDGVLDLSTKKSPCAGSTSLSHSPGCSSTQGNGRPGRPSQYRPDGLRSGDGVPPRSLQDGTREGFGHSTSLKVPLARSLQISEELLSRNQLSTAASLGPSGLQNHGQHLILSREASWAKPHYEFNLSRMKFRGNGALSNISDLPFLAENSAFPKMALQAKQDGKKDVSHSSPVDLKIPQVRGMDLSWESRTGDQYSYSSLVMGSQTESALSKKLRAILPKQSRKSMLDAGPDSWGSDAEQSTSGQPYPTSDQEGDPGSKQPRKKRGRYRQYNSEILEEAISVVMSGKMSVSKAQSIYGIPHSTLEYKVKERLGTLKNPPKKKMKLMSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ligand dependent nuclear receptor corepressor
别名 MLR2, Mblk1-related protein 2, Ligand-dependent corepressor, C10orf12
计算分子量 47 kDa
观测分子量 50 kDa
GenBank蛋白编号BC053359
基因名称 LCOR
Gene ID (NCBI) 84458
RRIDAB_2136862
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCOR is a transcriptional corepressor widely expressed in fetal and adult tissues that is recruited to agonist-bound nuclear receptors through a single LxxLL motif, also referred to as a nuclear receptor (NR) box [PMID:12535528]. It functions through recruitment of C-terminal binding proteins (CtBPs) and histone deacetylases (HDACs) to its N-terminal and central domains, respectively [PMID:22277651]. It is a scaffold for both histone deacetylases (HDACs) and C-terminal binding protein (CtBP) corepressors and functions by both HDAC-dependent and -independent mechanisms in a receptor-specific manner[PMID:19744932].

实验方案

Product Specific Protocols
IF protocol for LCOR antibody 14476-1-APDownload protocol
IHC protocol for LCOR antibody 14476-1-APDownload protocol
WB protocol for LCOR antibody 14476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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