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验证数据展示

  • WB analysis using 30222-1-AP (same clone as 30222-1-PBS)

    WB analysis using 30222-1-AP (same clone as 30222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30222-1-AP (LCMT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30222-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse uterus using 30222-1-AP (same clone as 30222-1-PBS)

    IHC staining of mouse uterus using 30222-1-AP (same clone as 30222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 30222-1-AP (LCMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LCMT1 antibody included

LCMT1 Polyclonal antibody, PBS Only

LCMT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30222-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® LCMT1 Ready-To-Use IHC Kit. LCMT1 primary antibody included.

产品信息

30222-1-PBS targets LCMT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32038

Product name: Recombinant human LCMT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 179-299 aa of BC001214

Sequence: EEKLKKCNMNTQLPTLLIAECVLVYMTPEQSANLLKWAANSFERAMFINYEQVNMGDRFGQIMIENLRRRQCDLAGVETCKSLESQKERLLSNGWETASAVDMMELYNRLPRAEVSRIESL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine carboxyl methyltransferase 1
别名
计算分子量 38 kDa
观测分子量 38-41 kDa
GenBank蛋白编号BC001214
基因名称 LCMT1
Gene ID (NCBI) 51451
RRIDAB_2935534
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIC8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Leucine carboxyl methyltransferase 1 (LCMT1) belongs to the methyltransferase superfamily (LCMT family). It methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. LCMT1 has 3 isoforms with the MW of 38 kDa, 41 kDa, and 32 kDa.

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