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验证数据展示

  • WB analysis using 30222-1-AP

    WB analysis using 30222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30222-1-AP (LCMT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse uterus using 30222-1-AP

    IHC staining of mouse uterus using 30222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 30222-1-AP (LCMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LCMT1 Polyclonal antibody

LCMT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30222-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

CGI 68, LCMT, LCMT1, PPMT1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cellls, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse uterus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30222-1-AP targets LCMT1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 LCMT1 fusion protein Ag32038 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine carboxyl methyltransferase 1
别名 CGI 68, LCMT, LCMT1, PPMT1
计算分子量 38 kDa
观测分子量 38-41 kDa
GenBank蛋白编号BC001214
基因名称 LCMT1
Gene ID (NCBI) 51451
RRIDAB_2935534
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIC8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine carboxyl methyltransferase 1 (LCMT1) belongs to the methyltransferase superfamily (LCMT family). It methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. LCMT1 has 3 isoforms with the MW of 38 kDa, 41 kDa, and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for LCMT1 antibody 30222-1-APDownload protocol
IHC protocol for LCMT1 antibody 30222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols