验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19333-1-AP targets LCA5 in WB, IF, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag5140 Product name: Recombinant human LCA5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 4-283 aa of BC050327 Sequence: RAGSPGTDQEREAGKHHYSYSSDFETPQSSGRSSLVSSSPASVRRKNPKRQTSDGQVHHQAPRKPSPKGLPNRKGVRVGFRSQSLNREPLRKDTDLVTKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQYRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEISEARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQRLYH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Leber congenital amaurosis 5 |
| 别名 | C6orf152, Leber congenital amaurosis 5, Lebercilin |
| 计算分子量 | 693 aa, 80 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC050327 |
| 基因名称 | LCA5 |
| Gene ID (NCBI) | 167691 |
| RRID | AB_2878576 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86VQ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LCA5 (Leber congenital amaurosis 5), also named as C6orf152 or Lebercilin, is a 697 amino acids protein. LCA5 is widely expressed at the microtubules, centrosome, and primary cilia. Recent study showed that LCA5 was involved in the cause of congenital and early-onset retinal dystrophies (PMID: 24144451). The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LCA5 antibody 19333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Amelioration of Neurosensory Structure and Function in Animal and Cellular Models of a Congenital Blindness. | ||
Hum Mol Genet Disruption of the Retinitis Pigmentosa 28 gene Fam161a in mice affects photoreceptor ciliary structure and leads to progressive retinal degeneration. | ||
Invest Ophthalmol Vis Sci Treatment Potential for LCA5-Associated Leber Congenital Amaurosis. | ||
J Proteomics Tissue- and isoform-specific protein complex analysis with natively processed bait proteins. | ||
Sci Rep The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system | ||
Nat Commun iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy |