×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 18130-1-AP (same clone as 18130-1-PBS)

    WB analysis using 18130-1-AP (same clone as 18130-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

    IP experiment of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

    IP result of anti-LC8/DYNLL1 (IP:18130-1-AP, 4ug; Detection:18130-1-AP 1:1000) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

    IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DYNLL1 antibody included
  • IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

    IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DYNLL1 antibody included
  • IF Staining of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

    IF Staining of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 18130-1-AP (same clone as 18130-1-PBS)

    FC experiment of Jurkat using 18130-1-AP (same clone as 18130-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

LC8/DYNLL1 Polyclonal antibody, PBS Only

LC8/DYNLL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 18130-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy DYNLL1 Ready-To-Use IHC Kit. DYNLL1 primary antibody included.

产品信息

18130-1-PBS targets LC8/DYNLL1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12710

Product name: Recombinant human LC8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-89 aa of BC100289

Sequence: MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, light chain, LC8-type 1
别名
计算分子量 89 aa, 10 kDa
观测分子量8-10 kDa
GenBank蛋白编号BC100289
基因名称 DYNLL1
Gene ID (NCBI) 8655
RRIDAB_2923569
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63167
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LC8 (also known as DYNLL1) is a homodimeric sequence-specific chaperone that promotes the ordered (typically homo-) oligomerization of more than a hundred protein targets. LC8/DYNLL1 has previously been proposed to function as an inhibitor of the NF-κB pathway, based on findings that it can bind directly to the IκBα regulatory region(PMID: 18579519). LC8/DYNLL1 regulates apoptotic activities of BCL2L11 by sequestering it to microtubules.

||
New chat

Able™

正在加载，请稍候...