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验证数据展示

  • WB analysis using 18130-1-AP

    WB analysis using 18130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 18130-1-AP

    IP experiment of MCF-7 using 18130-1-AP

    IP result of anti-LC8/DYNLL1 (IP:18130-1-AP, 4ug; Detection:18130-1-AP 1:1000) with MCF-7 cells lysate 640 ug.

  • IHC staining of mouse brain using 18130-1-AP

    IHC staining of mouse brain using 18130-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DYNLL1 antibody included
  • IHC staining of mouse brain using 18130-1-AP

    IHC staining of mouse brain using 18130-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DYNLL1 antibody included
  • IF Staining of MCF-7 using 18130-1-AP

    IF Staining of MCF-7 using 18130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 18130-1-AP

    FC experiment of Jurkat using 18130-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

LC8/DYNLL1 Polyclonal antibody

LC8/DYNLL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 18130-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

DYNLL1, Dynein light chain LC8 type 1, DNCLC1, DNCL1, DLC8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse cerebellum tissue, mouse testis tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy DYNLL1 Ready-To-Use IHC Kit. DYNLL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18130-1-AP targets LC8/DYNLL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag12710

Product name: Recombinant human LC8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-89 aa of BC100289

Sequence: MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, light chain, LC8-type 1
别名 DYNLL1, Dynein light chain LC8 type 1, DNCLC1, DNCL1, DLC8
计算分子量 89 aa, 10 kDa
观测分子量8-10 kDa
GenBank蛋白编号BC100289
基因名称 DYNLL1
Gene ID (NCBI) 8655
RRIDAB_2923569
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63167
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LC8 (also known as DYNLL1) is a homodimeric sequence-specific chaperone that promotes the ordered (typically homo-) oligomerization of more than a hundred protein targets. LC8/DYNLL1 has previously been proposed to function as an inhibitor of the NF-κB pathway, based on findings that it can bind directly to the IκBα regulatory region(PMID: 18579519). LC8/DYNLL1 regulates apoptotic activities of BCL2L11 by sequestering it to microtubules.

实验方案

Product Specific Protocols
WB protocol for LC8/DYNLL1 antibody 18130-1-APDownload protocol
IHC protocol for LC8/DYNLL1 antibody 18130-1-APDownload protocol
IF protocol for LC8/DYNLL1 antibody 18130-1-APDownload protocol
IP protocol for LC8/DYNLL1 antibody 18130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Authors - Ankur A Gholkar
View Article
mouseWB

Nanotoxicology

MiR-5622-3p inhibits ZCWPW1 to induce apoptosis in silica-exposed mice and spermatocyte cells

Authors - Moxuan Zhao
View Article