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验证数据展示

  • WB analysis of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

    WB analysis of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

    WB result of LC3B antibody (81004-1-RR; 1:20000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

    WB analysis of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

    IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LC3 antibody included
  • IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

    IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LC3 antibody included
  • IF Staining of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

    IF Staining of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HeLa cells using LC3B antibody (81004-1-RR, Clone: 5P12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81004-1-RR

    Affinity and Kinetic Characterization of 81004-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81004-1-RR against Human LC3 were performed. The affinity constant is 0.967 nM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

LC3B Recombinant monoclonal antibody, PBS Only

LC3B Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81004-1-PBS

产品说明书

CloneNo. 5P12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

LC3, MAP1LC3B, 5P12, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.

产品信息

81004-1-PBS targets LC3B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6144

Product name: Recombinant human LC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC067797

Sequence: MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMGELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein 1 light chain 3 beta
别名 LC3, MAP1LC3B, 5P12, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B
计算分子量 15 kDa
观测分子量14-18 kDa
GenBank蛋白编号BC067797
基因名称 LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9GZQ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. The antibody 81004-1-RR specifically recognizes LC3B.