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LC3 Recombinant antibody

LC3 Recombinant Antibody for WB, IHC, ELISA, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig and More (4)

Applications

WB, IHC, ELISA, IF/ICC and More (1)

Conjugate

Unconjugated

CloneNo.

5P12

Cat No : 81004-1-RR

Print datasheet

Synonyms

MAP1LC3B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B, Autophagy-related ubiquitin-like modifier LC3 B



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Chloroquine treated HEK-293 cells, Chloroquine treated HeLa cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inChloroquine treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy LC3 Ready-To-Use IHC Kit. LC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81004-1-RR targets LC3 in WB, IHC, ELISA, IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, ELISA, IF/ICC Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, mouse, rat, bovine
Immunogen LC3 fusion protein Ag6144 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name microtubule-associated protein 1 light chain 3 beta
Synonyms MAP1LC3B, MAP1A/1BLC3, MAP1 light chain 3-like protein 2, LC3B, Autophagy-related ubiquitin-like modifier LC3 B
Calculated Molecular Weight 15 kDa
Observed Molecular Weight14-18 kDa
GenBank Accession NumberBC067797
Gene Symbol LC3B
Gene ID (NCBI) 81631
ENSEMBL Gene IDENSG00000140941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.

实验方案

Product Specific Protocols
WB protocol for LC3 antibody 81004-1-RRDownload protocol
IHC protocol for LC3 antibody 81004-1-RRDownload protocol
IF protocol for LC3 antibody 81004-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB

J Dairy Sci

Subacute ruminal acidosis induces pyroptosis via the mitophagy-mediated NLRP3 inflammasome activation in the livers of dairy cows fed a high-grain diet

Authors - Hongzhu Zhang
humanWB

Discov Oncol

L-cysteine contributes to destructive activities of odontogenic cysts/tumor

Authors - Ji Li
mouseWB,IF

Int J Mol Sci

Combination of Kaempferol and Docetaxel Induces Autophagy in Prostate Cancer Cells In Vitro and In Vivo

Authors - Qian Zhou
rat

J Hazard Mater

The role of mTORC1/TFEB axis mediated lysosomal biogenesis and autophagy impairment in fluoride neurotoxicity and the intervention effects of resveratrol

Authors - Huayang Tang
mouseWB

Acta Biomater

Tumor-targeted Delivery of Copper-manganese Biomineralized Oncolytic Adenovirus for Colorectal Cancer Immunotherapy

Authors - Yi-Shu Li