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LBP Polyclonal antibody

LBP Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11836-1-AP

Print datasheet

Synonyms

LBP

验证数据展示

  • WB analysis of mouse liver using 11836-1-AP

    WB analysis of mouse liver using 11836-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 11836-1-AP

    IF Staining of L02 using 11836-1-AP

    Immunofluorescent analysis of L02 cells using 11836-1-AP (LBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse liver using 11836-1-AP

    IP experiment of mouse liver using 11836-1-AP

    IP result of anti-LBP (IP:11836-1-AP, 4ug; Detection:11836-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

  • FC experiment of HeLa using 11836-1-AP

    FC experiment of HeLa using 11836-1-AP

    1X10^6 HeLa cells were stained with 0.2ug LBP antibody (11836-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • IHC staining of human liver using 11836-1-AP

    IHC staining of human liver using 11836-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with LBP antibody included
  • IHC staining of human liver using 11836-1-AP

    IHC staining of human liver using 11836-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with LBP antibody included

经过测试的应用

Positive WB detected inmouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells, HepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LBP Ready-To-Use IHC Kit. LBP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11836-1-AP targets LBP in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen LBP fusion protein Ag2431 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipopolysaccharide binding protein
Synonyms LBP
Calculated Molecular Weight 481 aa, 53 kDa
Observed Molecular Weight 63-67 kDa
GenBank Accession NumberBC022256
Gene Symbol LBP
Gene ID (NCBI) 3929
RRIDAB_2097479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60kd and 63-67kd(Glycosylation form).

实验方案

Product Specific Protocols
WB protocol for LBP antibody 11836-1-APDownload protocol
IHC protocol for LBP antibody 11836-1-APDownload protocol
IF protocol for LBP antibody 11836-1-APDownload protocol
IP protocol for LBP antibody 11836-1-APDownload protocol
FC protocol for LBP antibody 11836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Neurosurg

Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.

Authors - Nataliya Smith
View Article
humanWB

Cancer Sci

Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.

Authors - Ning Wang
View Article
ratIHC

J Biomed Mater Res A

The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.

Authors - Takafumi Kobayashi
View Article