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验证数据展示

  • WB analysis using 82491-1-RR (same clone as 82491-1-PBS)

    WB analysis using 82491-1-RR (same clone as 82491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82491-1-RR (LASP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82491-1-RR (same clone as 82491-1-PBS)

    IHC staining of human colon using 82491-1-RR (same clone as 82491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82491-1-RR (LASP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LASP1 antibody included
  • IHC staining of human stomach using 82491-1-RR (same clone as 82491-1-PBS)

    IHC staining of human stomach using 82491-1-RR (same clone as 82491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82491-1-RR (LASP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LASP1 antibody included
  • IF Staining of HeLa using 82491-1-RR (same clone as 82491-1-PBS)

    IF Staining of HeLa using 82491-1-RR (same clone as 82491-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LASP1 antibody (82491-1-RR, Clone: 4J2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

LASP1 Recombinant monoclonal antibody, PBS Only

LASP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82491-1-PBS

产品说明书

CloneNo. 4J2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

4J2, LASP-1, LIM and SH3 protein 1, MLN50

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.

产品信息

82491-1-PBS targets LASP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0800

Product name: Recombinant human LASP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 125-210 aa of BC012460

Sequence: EFEKSRMGPSGGEGMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSYGGYKEPAAPVSIQRSAPGGGGKRYRAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 LIM and SH3 protein 1
别名 4J2, LASP-1, LIM and SH3 protein 1, MLN50
计算分子量 30 kDa
观测分子量 35 kDa
GenBank蛋白编号BC012460
基因名称 LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14847
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

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