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验证数据展示

  • WB analysis of Jurkat using 22441-1-AP (same clone as 22441-1-PBS)

    WB analysis of Jurkat using 22441-1-AP (same clone as 22441-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

    IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARHGEF12/LARG antibody included
  • IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

    IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARHGEF12/LARG antibody included
  • IF Staining of HepG2 using 22441-1-AP (same clone as 22441-1-PBS)

    IF Staining of HepG2 using 22441-1-AP (same clone as 22441-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 22441-1-AP (same clone as 22441-1-PBS)

    IP experiment of HEK-293 using 22441-1-AP (same clone as 22441-1-PBS)

    IP result of anti-LARG (IP:22441-1-AP, 4ug; Detection:22441-1-AP 1:4000) with HEK-293 cells lysate 1320 ug. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LARG Polyclonal antibody, PBS Only

LARG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22441-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ARHGEF12, KIAA0382, Leukemia associated RhoGEF, Leukemia-associated RhoGEF

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ARHGEF12/LARG Ready-To-Use IHC Kit. ARHGEF12/LARG primary antibody included.

产品信息

22441-1-PBS targets LARG in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18211

Product name: Recombinant human LARG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 214-563 aa of BC063117

Sequence: QRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho guanine nucleotide exchange factor (GEF) 12
别名 ARHGEF12, KIAA0382, Leukemia associated RhoGEF, Leukemia-associated RhoGEF
计算分子量 1544 aa, 173 kDa
观测分子量 220 kDa
GenBank蛋白编号BC063117
基因名称 LARG
Gene ID (NCBI) 23365
RRIDAB_2879107
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZN5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.

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