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验证数据展示

  • FC experiment of human monocyte-derived dendritic using 98408-3-RR

    FC experiment of human monocyte-derived dendritic using 98408-3-RR

    1x10^6 unstimulated human CD14+ monocytes or human monocyte-derived dendritic cells were surface stained with 0.25 ug Anti-Human LAMP3 Rabbit RecAb (98408-3-RR, Clone: 242748F3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of human monocyte-derived dendritic using 98408-3-RR

    FC experiment of human monocyte-derived dendritic using 98408-3-RR

    1x10^6 human monocyte-derived dendritic cells were surface stained with 0.25 ug Anti-Human LAMP3/CD208 Rabbit RecAb (98408-3-RR, Clone: 242748F3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human CD14+ monocytes were surface stained with 0.25 ug Anti-Human LAMP3/CD208 Rabbit RecAb (98408-3-RR, Clone: 242748F3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

Anti-Human LAMP3 Rabbit Recombinant Antibody

LAMP3 Uni-rAb® Recombinant Antibody for FC
Cat No. 98408-3-RR

产品说明书

CloneNo. 242748F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD208, DC LAMP, DCLAMP, DC-lysosome-associated membrane glycoprotein, LAMP

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman monocyte-derived dendritic cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98408-3-RR targets LAMP3 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg3594

Product name: Recombinant Human LAMP3 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-381 aa of NM_014398.4

Sequence: KAFPETRDYSQPTAAATVQDIKKPVQQPAKQAPHQTLAARFMDGHITFQTAATVKIPTTTPATTKNTATTSPITYTLVTTQATPNNSHTAPPVTEVTVGPSLAPYSLPPTITPPAHTTGTSSSTVSHTTGNTTQPSNQTTLPATLSIALHKSTTGQKPVQPTHAPGTTAAAHNTTRTAAPASTVPGPTLAPQPSSVKTGIYQVLNGSRLCIKAEMGIQLIVQDKESVFSPRRYFNIDPNATQASGNCGTRKSNLLLNFQGGFVNLTFTKDEESYYISEVGAYLTVSDPETIYQGIKHAVVMFQTAVGHSFKCVSEQSLQLSAHLQVKTTDVQLQAFDFEDDHFGNVDECSSDYT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 3
别名 CD208, DC LAMP, DCLAMP, DC-lysosome-associated membrane glycoprotein, LAMP
计算分子量44 kDa
GenBank蛋白编号NM_014398.4
基因名称 LAMP3
Gene ID (NCBI) 27074
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UQV4
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403, or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. LAMP3 is expressed in lung type II pneumocytes, lymphoid organs, and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells (PMID:9721848).

实验方案

Product Specific Protocols
FC protocol for LAMP3 antibody 98408-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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