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验证数据展示

  • WB analysis of DU 145 using 20928-1-AP

    WB analysis of DU 145 using 20928-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of DU 145 using 20928-1-AP

    IP experiment of DU 145 using 20928-1-AP

    IP result of anti-LAMC1 (IP:20928-1-AP, 4ug; Detection:20928-1-AP 1:1000) with DU 145 cells lysate 3600ug.

  • WB analysis of HeLa using 20928-1-AP

    WB analysis of HeLa using 20928-1-AP

    WB result of LAMC1 antibody (20928-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAMC1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

LAMC1 Polyclonal antibody

LAMC1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20928-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

Laminin subunit gamma 1, LAMB2, LAMC 1, Laminin subunit gamma-1, Laminin-1 subunit gamma

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells
Positive IP detected inDU 145 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20928-1-AP targets LAMC1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14674

Product name: Recombinant human LAMC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1260-1609 aa of BC015586

Sequence: AGDKAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELEVKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVNDNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAVQKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 laminin, gamma 1 (formerly LAMB2)
别名 Laminin subunit gamma 1, LAMB2, LAMC 1, Laminin subunit gamma-1, Laminin-1 subunit gamma
计算分子量 178 kDa
观测分子量 220 kDa, 178 kDa
GenBank蛋白编号BC015586
基因名称 LAMC1
Gene ID (NCBI) 3915
RRIDAB_2878767
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11047
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for LAMC1 antibody 20928-1-APDownload protocol
WB protocol for LAMC1 antibody 20928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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