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验证数据展示

  • FC experiment of human PBMCs using 98435-3-RR

    FC experiment of human PBMCs using 98435-3-RR

    1x10^6 untreated or anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-3-RR, Clone:243086H8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (CL647-65559, Clone: UCHT4). Cells were not fixed.

  • IHC staining of human tonsillitis using 98435-3-RR

    IHC staining of human tonsillitis using 98435-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98435-3-RR (LAG3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 98435-3-RR

    IHC staining of human tonsillitis using 98435-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98435-3-RR (LAG3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of human PBMCs using 98435-3-RR

    FC experiment of human PBMCs using 98435-3-RR

    1x10^6 anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-3-RR, Clone: 243086H8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human LAG3 Rabbit RecAb (98435-3-RR, Clone: 243086H8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  • Affinity of 98435-3-RR

    Affinity of 98435-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98435-3-RR against Human LAG-3/CD223 were performed. The affinity constant is 0.889 nM.

Anti-Human LAG-3/CD223 Rabbit Recombinant Antibody

LAG-3/CD223 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98435-3-RR

产品说明书

CloneNo. 243086H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

CD223, LAG3, FDC, LAG 3, LAG-3

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
FITC Plus
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inanti-CD3/CD28 and IL-2 treated human PBMCs

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98435-3-RR targets LAG-3/CD223 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg31550

Product name: Recombinant Human LAG-3/CD223protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 23-450 aa of NM_002286

Sequence: LQPGAEVPVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSCRLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPHHHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITVTPKSFGSPGSLGKLLCEVTPVSGQERFVWSSLDTPSQRSFSGPWLEAQEAQLLSQPWQCQLYQGERLLGAAVYFTELSSPGAQRSGRAPGALPAGHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte-activation gene 3
别名 CD223, LAG3, FDC, LAG 3, LAG-3
计算分子量57 kDa
GenBank蛋白编号NM_002286
基因名称 LAG-3
Gene ID (NCBI) 3902
RRIDAB_3746073
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18627
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

实验方案

Product Specific Protocols
FC protocol for LAG-3/CD223 antibody 98435-3-RRDownload protocol
IHC protocol for LAG-3/CD223 antibody 98435-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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