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验证数据展示

  • WB analysis of A549 using 67399-1-Ig

    WB analysis of A549 using 67399-1-Ig

    WB result of LACTB2 antibody (67399-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

  • WB analysis using 67399-1-Ig

    WB analysis using 67399-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67399-1-Ig

    IF Staining of A549 using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

  • IHC staining of human liver cancer using 67399-1-Ig

    IHC staining of human liver cancer using 67399-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67399-1-Ig

    IHC staining of human liver cancer using 67399-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 67399-1-Ig

    IF Staining of human liver cancer using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67399-1-Ig

    IF Staining of human liver cancer using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse liver using 67399-1-Ig

    IF Staining of mouse liver using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

LACTB2 Monoclonal antibody

LACTB2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67399-1-Ig

产品说明书

CloneNo. 1G6B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

EC:3.1.27.-, CGI-83, CGI83, CGI 83, Beta-lactamase-like protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, mouse liver tissue
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67399-1-Ig targets LACTB2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10231

Product name: Recombinant human LACTB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-288 aa of BC000878

Sequence: MAAVLQRVERLSNRVVRVLGCNPGPMTLQGTNTYLVGTGPRRILIDTGEPAIPEYISCLKQALTEFNTAIQEIVVTHWHRDHSGGIGDICKSINNDTTYCIKKLPRNPQREEIIGNGEQQYVYLKDGDVIKTEGATLRVLYTPGHTDDHMALLLEEENAIFSGDCILGEGTTVFEDLYDYMNSLKELLKIKADIIYPGHGPVIHNAEAKIQQYISHRNIREQQILTLFRENFEKSFTVMELVKIIYKNTPENLHEMAKHNLLLHLKKLEKEGKIFSNTDPDKKWKAHL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lactamase, beta 2
别名 EC:3.1.27.-, CGI-83, CGI83, CGI 83, Beta-lactamase-like protein 2
计算分子量 288 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC000878
基因名称 LACTB2
Gene ID (NCBI) 51110
RRIDAB_2882642
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ53H82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for LACTB2 antibody 67399-1-IgDownload protocol
IHC protocol for LACTB2 antibody 67399-1-IgDownload protocol
WB protocol for LACTB2 antibody 67399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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