• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

LACTB2 Polyclonal antibody

LACTB2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16783-1-AP

Print datasheet

Synonyms

Beta lactamase like protein 2, CGI 83, lactamase, beta 2, LACTB2

验证数据展示

  • WB analysis of A549 using 16783-1-AP

    WB analysis of A549 using 16783-1-AP

    WB result of LACTB2 antibody (16783-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

  • WB analysis using 16783-1-AP

    WB analysis using 16783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervix using 16783-1-AP

    IHC staining of human cervix using 16783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human cervix using 16783-1-AP

    IHC staining of human cervix using 16783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16783-1-AP

    IF Staining of HepG2 using 16783-1-AP

    Immunofluorescent analysis of HepG2 cells, using LACTB2 antibody 16783-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A549 using 16783-1-AP

    IP experiment of A549 using 16783-1-AP

    IP result of anti-LACTB2 (IP:16783-1-AP, 3ug; Detection:16783-1-AP 1:700) with A549 cells lysate 520ug.

经过测试的应用

Positive WB detected inMCF-7 cells, PC-3 cells, Y79 cells, A549 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16783-1-AP targets LACTB2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen LACTB2 fusion protein Ag10213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lactamase, beta 2
Synonyms Beta lactamase like protein 2, CGI 83, lactamase, beta 2, LACTB2
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000878
Gene Symbol LACTB2
Gene ID (NCBI) 51110
RRIDAB_2133333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53H82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LACTB2 antibody 16783-1-APDownload protocol
IHC protocol for LACTB2 antibody 16783-1-APDownload protocol
IF protocol for LACTB2 antibody 16783-1-APDownload protocol
IP protocol for LACTB2 antibody 16783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols