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验证数据展示

  • WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human colon cancer using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with L1CAM antibody included
  • IHC staining of human colon cancer using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human colon cancer using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with L1CAM antibody included
  • IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human colon using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with L1CAM antibody included

L1CAM/CD171 Monoclonal antibody, PBS Only

L1CAM/CD171 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67115-1-PBS

产品说明书

CloneNo. 3H7B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

L1CAM, 3H7B9, CAML1, CD171, HSAS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

产品信息

67115-1-PBS targets L1CAM/CD171 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag17706

Product name: Recombinant human L1CAM protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 794-1143 aa of BC126229

Sequence: QGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 L1CAM, 3H7B9, CAML1, CD171, HSAS
计算分子量 1257 aa, 140 kDa
观测分子量 220 kDa
GenBank蛋白编号BC126229
基因名称 L1CAM
Gene ID (NCBI) 3897
RRIDAB_2882419
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP32004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

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