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验证数据展示

  • WB analysis of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    WB analysis of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 86143-1-RR (Klk1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    IHC staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 86143-1-RR (Klk1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    IHC staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 86143-1-RR (Klk1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    IF Staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Klk1 antibody (86143-1-RR, Clone: 250034D2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    IF Staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Klk1 antibody (86143-1-RR, Clone: 250034D2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

Mouse Klk1 Recombinant monoclonal antibody, PBS Only

Klk1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86143-1-PBS

产品说明书

CloneNo. 250034D2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

Kallikrein 1, EC:3.4.21.35, Glandular kallikrein K1, KAL-B, Kallikrein-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86143-1-PBS targets Klk1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2996

Product name: recombinant mouse Klk1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-261 aa of NM_001320331.1

Sequence: PPVQSRIVGGFNCEKNSQPWQVAVYRFTKYQCGGILLNANWVLTAAHCHNDKYQVWLGKNNFLEDEPSAQHRLVSKAIPHPDFNMSLLNEHTPQPEDDYSNDLMLLRLKKPADITDVVKPIDLPTEEPKLGSTCLASGWGSITPVKYEYPDELQCVNLKLLPNEDCAKAHIEKVTDDMLCAGDMDGGKDTCAGDSGGPLICDGVLQGITSWGPSPCGKPNVPGIYTRVLNFNTWIRETMAEND

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kallikrein 1
别名 Kallikrein 1, EC:3.4.21.35, Glandular kallikrein K1, KAL-B, Kallikrein-1
计算分子量29 kDa
观测分子量28-34 kDa
GenBank蛋白编号NM_001320331.1
基因名称 Klk1
Gene ID (NCBI) 16612
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15947
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mouse KLK1 (Kallikrein-Related Peptidase 1) is a serine protease belonging to the kallikrein family with important physiological functions. KLK1 can cleave the Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Bradykinin plays an important role in regulating vasodilation, lowering blood pressure, smooth muscle relaxation and contraction, pain induction and inflammatory response.

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