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验证数据展示

  • WB analysis using 27477-1-AP

    WB analysis using 27477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27477-1-AP (Kininogen 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27477-1-AP

    IHC staining of human kidney using 27477-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 27477-1-AP

    IHC staining of human kidney using 27477-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens).

Kininogen 1 Polyclonal antibody

Kininogen 1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27477-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27477-1-AP targets Kininogen 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26467

Product name: Recombinant human KNG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-200 aa of BC060039

Sequence: ESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kininogen 1
别名 BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1
计算分子量 644 aa, 72 kDa
观测分子量 70-72 kDa
GenBank蛋白编号BC060039
基因名称 Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_2880882
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

实验方案

Product Specific Protocols
IHC protocol for Kininogen 1 antibody 27477-1-APDownload protocol
WB protocol for Kininogen 1 antibody 27477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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