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验证数据展示

  • FC experiment of human PBMCs using CL647-98143

    FC experiment of human PBMCs using CL647-98143

    1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with PE Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human Ki-67 Rabbit RecAb (CL647-98143, Clone: 241499B1). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of human PBMCs using CL647-98143

    FC experiment of human PBMCs using CL647-98143

    1x10^6 PHA-treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human Ki-67 Rabbit RecAb (CL647-98143, Clone: 241499B1) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human Ki-67 Rabbit RecAb (CL647-98143, Clone: 241499B1) (black, dashed). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647 Anti-Human Ki-67 Rabbit Recombinant Antibody

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL647-98143

产品说明书

CloneNo. 241499B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

KI67, MKI67, 241499B1, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98143 targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki-67
别名 KI67, MKI67, 241499B1, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67
计算分子量 359 kDa
GenBank蛋白编号NM_002417
基因名称 KI67
Gene ID (NCBI) 4288
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46013
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Ki-67 antibody CL647-98143Download protocol
Standard Protocols
Click here to view our Standard Protocols