CoraLite®594-conjugated Ki-67 Recombinant antibody

Ki-67 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL594-84192-4

产品说明书

CloneNo. 241499E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

KI67, MKI67, 241499E7, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84192-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-84192-4 targets Ki-67 in applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki-67
别名 KI67, MKI67, 241499E7, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67
计算分子量 359 kDa
GenBank蛋白编号NM_002417
基因名称 KI67
Gene ID (NCBI) 4288
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP46013
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
IF protocol for CL594 Ki-67 antibody CL594-84192-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...