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- KD/KO Validated
CoraLite® Plus 647-conjugated KU70 Polyclonal antibody
KU70 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy XRCC6 Ready-To-Use IHC Kit. XRCC6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10723-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-10723 targets KU70 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | human, rat, mouse |
| Immunogen | KU70 fusion protein Ag1043 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Synonyms | 5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, ML8, Thyroid lupus autoantigen, TLAA, XRCC6 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC008343 |
| Gene Symbol | XRCC6 |
| Gene ID (NCBI) | 2547 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12956 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 KU70 antibody CL647-10723 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |