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验证数据展示

  • WB analysis using 32552-1-AP

    WB analysis using 32552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32552-1-AP (KREMEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

KREMEN1 Polyclonal antibody

KREMEN1 Polyclonal Antibody for WB, ELISA
Cat No. 32552-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Kringle-containing protein marking the eye and the nose, Kringle domain-containing transmembrane protein 1, Kremen protein 1, KREMEN, KREMEM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32552-1-AP targets KREMEN1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 KREMEN1 fusion protein Ag37569 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kringle containing transmembrane protein 1
别名 Kringle-containing protein marking the eye and the nose, Kringle domain-containing transmembrane protein 1, Kremen protein 1, KREMEN, KREMEM1
计算分子量 473 aa, 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC063787
基因名称 KREMEN1
Gene ID (NCBI) 83999
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96MU8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kremen protein 1 (KREMEN1, also known as KRM1) is a transmembrane protein that is a negative regulator of the Wnt signaling pathway (PMID: 26206087; 18502762). KREMEN1 is one of the alternative functional receptors that play essential roles in ACE2-independent virus entry, providing insight into SARS-CoV-2 tropism and pathogenesis, as well as a community resource and potential therapeutic strategies for further COVID-19 investigations (PMID: 34837059; 34903854).

实验方案

Product Specific Protocols
WB protocol for KREMEN1 antibody 32552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols