验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32552-1-AP targets KREMEN1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37569 Product name: Recombinant human KREMEN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 100-370 aa of BC063787 Sequence: AEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKTSNKLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKYGEAASTECNSVCFGDHTQPCGGDGRIILFDTLVGACGGNYSAMSSVVYSPDFPDTYATGRVCYWTIRVPGASHIHFSFPLFDIRDSADMVELLDGYTHRVLARFHGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQAVKEELPQERPAVNQTVAEVITEQANLSVSAARSSKVLYVITTSPSHPP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kringle containing transmembrane protein 1 |
| 别名 | Kringle-containing protein marking the eye and the nose, Kringle domain-containing transmembrane protein 1, Kremen protein 1, KREMEN, KREMEM1 |
| 计算分子量 | 473 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC063787 |
| 基因名称 | KREMEN1 |
| Gene ID (NCBI) | 83999 |
| RRID | AB_3742654 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96MU8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kremen protein 1 (KREMEN1, also known as KRM1) is a transmembrane protein that is a negative regulator of the Wnt signaling pathway (PMID: 26206087; 18502762). KREMEN1 is one of the alternative functional receptors that play essential roles in ACE2-independent virus entry, providing insight into SARS-CoV-2 tropism and pathogenesis, as well as a community resource and potential therapeutic strategies for further COVID-19 investigations (PMID: 34837059; 34903854).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KREMEN1 antibody 32552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
