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验证数据展示

  • WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

    WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

    WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IP experiment of MKN-45 using 12063-1-AP (same clone as 12063-1-PBS)

    IP experiment of MKN-45 using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:8000) with MKN-45 cells lysate 1560 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IP experiment of HGC-27 using 12063-1-AP (same clone as 12063-1-PBS)

    IP experiment of HGC-27 using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:8000) with HGC-27 cells lysate 1520 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    IP experiment of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:20000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:20000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 12063-1-AP (same clone as 12063-1-PBS)

    IHC staining of mouse kidney using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KRAS Polyclonal antibody, PBS Only

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12063-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

pan RAS, C K RAS, c Ki ras, c-Ki-ras, c-K-ras

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12063-1-PBS targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2700

Product name: Recombinant human KRAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-188 aa of BC013572

Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
别名 pan RAS, C K RAS, c Ki ras, c-Ki-ras, c-K-ras
计算分子量 188 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC013572
基因名称 KRAS
Gene ID (NCBI) 3845
RRIDAB_878040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01116
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

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