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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of BxPC-3 using 12366-2-AP

    WB analysis of BxPC-3 using 12366-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12366-2-AP (KPNA6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12366-2-AP

    IHC staining of human breast cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA6 antibody included
  • IHC staining of human breast cancer using 12366-2-AP

    IHC staining of human breast cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA6 antibody included

KPNA6 Polyclonal antibody

KPNA6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12366-2-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KPNA6 Ready-To-Use IHC Kit. KPNA6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12366-2-AP targets KPNA6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 KPNA6 fusion protein Ag3040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 karyopherin alpha 6 (importin alpha 7)
别名 Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6
计算分子量 536 aa, 60 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC020520
基因名称 KPNA6
Gene ID (NCBI) 23633
RRIDAB_2281067
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60684
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KPNA6 antibody 12366-2-APDownload protocol
IHC protocol for KPNA6 antibody 12366-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Authors - Sergi Marco
View Article
humanWB

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
View Article
humanWB

Viruses

Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2

Authors - Anna Nakanishi
View Article