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KPNA6 Polyclonal antibody

KPNA6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 12366-2-AP

Print datasheet

Synonyms

Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6

验证数据展示

  • WB analysis of BxPC-3 using 12366-2-AP

    WB analysis of BxPC-3 using 12366-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12366-2-AP (KPNA6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12366-2-AP

    IHC staining of human breast cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 12366-2-AP

    IHC staining of human breast cancer using 12366-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12366-2-AP (KPNA6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12366-2-AP targets KPNA6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen KPNA6 fusion protein Ag3040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name karyopherin alpha 6 (importin alpha 7)
Synonyms Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6
Calculated Molecular Weight 536 aa, 60 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC020520
Gene Symbol KPNA6
Gene ID (NCBI) 23633
RRIDAB_2281067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KPNA6 antibody 12366-2-APDownload protocol
IHC protocol for KPNA6 antibody 12366-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Authors - Sergi Marco
View Article
humanWB

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
View Article
humanWB

Viruses

Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2

Authors - Anna Nakanishi
View Article