• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of MCF-7 using 83595-4-RR (same clone as 83595-4-PBS)

    IF Staining of MCF-7 using 83595-4-RR (same clone as 83595-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMT2C antibody (83595-4-RR, Clone: 240542A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (green). This data was developed using the same antibody clone with 83595-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83595-4-RR (same clone as 83595-4-PBS)

    FC experiment of U2OS using 83595-4-RR (same clone as 83595-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug KMT2C Recombinant antibody (83595-4-RR, Clone:240542A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83595-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83595-4-RR

    Affinity and Kinetic Characterization of 83595-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83595-4-RR against Human KMT2C were performed. The affinity constant is 2.67 nM.

KMT2C Recombinant antibody, PBS Only

KMT2C Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83595-4-PBS

产品说明书

CloneNo. 240542A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

MLL3, 240542A2, HALR, Homologous to ALR protein, KIAA1506

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83595-4-PBS targets KMT2C in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 KMT2C fusion protein Ag28875 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myeloid/lymphoid or mixed-lineage leukemia 3
别名 MLL3, 240542A2, HALR, Homologous to ALR protein, KIAA1506
计算分子量 541 kDa
GenBank蛋白编号NM_170606
基因名称 KMT2C
Gene ID (NCBI) 58508
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NEZ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.