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验证数据展示

  • IF Staining of Jurkat using CL488-84785-5

    IF Staining of Jurkat using CL488-84785-5

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 KLRG1 antibody (CL488-84785-5, Clone: 242327A4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated KLRG1 Recombinant antibody

KLRG1 Recombinant Antibody for IF/ICC
Cat No. CL488-84785-5

产品说明书

CloneNo. 242327A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

242327A4, CLEC15A, C-type lectin domain family 15 member A, ITIM-containing receptor MAFA-L, Killer cell lectin-like receptor subfamily G member 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 84785-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84785-5 targets KLRG1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 KLRG1 fusion protein Eg2036 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 killer cell lectin-like receptor subfamily G, member 1
别名 242327A4, CLEC15A, C-type lectin domain family 15 member A, ITIM-containing receptor MAFA-L, Killer cell lectin-like receptor subfamily G member 1
计算分子量22 kDa
GenBank蛋白编号NM_005810.4
基因名称 KLRG1
Gene ID (NCBI) 10219
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96E93-1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KLRG1 antibody CL488-84785-5Download protocol
Standard Protocols
Click here to view our Standard Protocols