验证数据展示
经过测试的应用
| Positive WB detected in | mouse skin tissue, rat skin tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23810-1-AP targets Kallikrein 7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag20760 Product name: Recombinant human KLK7 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-253 aa of BC032005 Sequence: MARSLLLPLQILLLSLALETAGEEAQGDKIIDGAPCARGSHPWQVALLSGNQLHCGGVLVNERWVLTAAHCKMNEYTVHLGSDTLGDRRAQRIKASKSFRHPGYSTQTHVNDLMLVKLNSQARLSSMVKKVRLPSRCEPPGTTCTVSGWGTTTSPDVTFPSDLMCVDVKLISPQDCTKVYKDLLENSMLCAGIPDSKKNACNGDSGGPLVCRGTLQGLVSWGTFPWGQPNDPGVYTQVCKFTKWINDTMKKHR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kallikrein-related peptidase 7 |
| 别名 | KLK7, EC:3.4.21.117, Kallikrein-7, PRSS6, SCCE |
| 计算分子量 | 28 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC032005 |
| 基因名称 | KLK7 |
| Gene ID (NCBI) | 5650 |
| RRID | AB_3669417 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P49862 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kallikrein-7(KLK7), also known as SCCE, PRSS6, is a member of the kallikrein subfamily of serine proteases involved in various enzymatic processes. KLK7 is abundantly expressed in the skin and is expressed by keratinocytes in the epidermis(PMID: 10974542).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Kallikrein 7 antibody 23810-1-AP | Download protocol |
| WB protocol for Kallikrein 7 antibody 23810-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
