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验证数据展示

  • WB analysis using 60338-1-Ig (same clone as 60338-1-PBS)

    WB analysis using 60338-1-Ig (same clone as 60338-1-PBS)

    PC-3(PSA-), DU 145(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSA antibody included
  • IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    IHC staining of human prostate cancer using 60338-1-Ig (same clone as 60338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60338-1-Ig (KLK3/PSA antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSA antibody included

KLK3/PSA Monoclonal antibody, PBS Only

KLK3/PSA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60338-1-PBS

产品说明书

CloneNo. 4A1E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

KLK3, 4A1E2, APS, EC:3.4.21.77, Gamma-seminoprotein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.

产品信息

60338-1-PBS targets KLK3/PSA in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1060

Product name: Recombinant human KLK3,PSA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-261 aa of BC005307

Sequence: MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 3
别名 KLK3, 4A1E2, APS, EC:3.4.21.77, Gamma-seminoprotein
计算分子量 29 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC005307
基因名称 KLK3
Gene ID (NCBI) 354
RRIDAB_2881447
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07288
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.

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