• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 10679-1-AP

    WB analysis using 10679-1-AP

    DU 145(PSA-), PC-3(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10679-1-AP

    IHC staining of human prostate cancer using 10679-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSA antibody included
  • IHC staining of human prostate cancer using 10679-1-AP

    IHC staining of human prostate cancer using 10679-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSA antibody included
  • IP experiment of LNCaP using 10679-1-AP

    IP experiment of LNCaP using 10679-1-AP

    IP result of anti-KLK3/PSA (IP:10679-1-AP, 4ug; Detection:10679-1-AP 1:10000) with LNCaP cells lysate 1520 ug.

  • IHC staining of human prostate cancer using 10679-1-AP

    IHC staining of human prostate cancer using 10679-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSA antibody included
  • IHC staining of human prostate cancer using 10679-1-AP

    IHC staining of human prostate cancer using 10679-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSA antibody included
  • IF Staining of mouse prostate using 10679-1-AP

    IF Staining of mouse prostate using 10679-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse prostate tissue using KLK3/PSA antibody (10679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of LNCaP using 10679-1-AP

    IF Staining of LNCaP using 10679-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using KLK3/PSA antibody (10679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

KLK3/PSA Polyclonal antibody

KLK3/PSA Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 10679-1-AP

产品说明书

引用文献(30)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

KLK3, APS, EC:3.4.21.77, Gamma-seminoprotein, Kallikrein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, Transfected HEK-293 cells
Positive IP detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse prostate tissue
Positive IF/ICC detected inLNCaP cells
Planning an IHC experiment? We recommend our IHCeasy PSA Ready-To-Use IHC Kit. PSA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10679-1-AP targets KLK3/PSA in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag1060

Product name: Recombinant human KLK3,PSA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-261 aa of BC005307

Sequence: MWVPVVFLTLSVTWIGAAPLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kallikrein-related peptidase 3
别名 KLK3, APS, EC:3.4.21.77, Gamma-seminoprotein, Kallikrein 3
计算分子量 29 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC005307
基因名称 KLK3
Gene ID (NCBI) 354
RRIDAB_2134244
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07288
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK3 also known as Prostate-Specific Antigen (PSA), is a 33 kDa glycoprotein primarily synthesized by the epithelial cells of the prostate gland. Its main physiological function is to cleave seminogelins I and II in semen, leading to the liquefaction of the seminal coagulum and the release of motile sperm. It is under tight regulation by androgens via the androgen receptor (AR). Serum PSA levels are elevated in prostate cancer due to disruption of the glandular architecture, allowing more PSA to enter the bloodstream. It is used alongside digital rectal exam (DRE) for early detection. Although termed "prostate-specific," PSA is not exclusively produced by the prostate. Very low levels can be produced in periurethral glands, breast tissue, and some other tissues. Its expression is highly abundant in prostatic tissue and seminal fluid.

实验方案

Product Specific Protocols
IF protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IHC protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IP protocol for KLK3/PSA antibody 10679-1-APDownload protocol
WB protocol for KLK3/PSA antibody 10679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIHC,IF

Nat Aging

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Authors - Yifei Cheng
View Article
humanIHC

Theranostics

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.

Authors - Lijie Zhou
View Article
mouseIHC

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
View Article
humanWB

J Transl Med

CAPN2 promotes apalutamide resistance in metastatic hormone-sensitive prostate cancer by activating protective autophagy

Authors - Zihao Qi
View Article
humanWB

Elife

A feedback loop between the androgen receptor and 6-phosphogluoconate dehydrogenase (6PGD) drives prostate cancer growth

Authors - Joanna L Gillis
View Article
humanWB

Mol Cancer Ther

PMID: 28802253

Authors - Linh N K Tran
View Article
||
New chat Able
正在加载，请稍候...