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验证数据展示

  • WB analysis using 28621-1-AP (same clone as 28621-1-PBS)

    WB analysis using 28621-1-AP (same clone as 28621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28621-1-AP (KLHL32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28621-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28621-1-AP (same clone as 28621-1-PBS)

    IHC staining of mouse brain using 28621-1-AP (same clone as 28621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28621-1-AP (KLHL32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28621-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 28621-1-AP (same clone as 28621-1-PBS)

    IF Staining of U2OS using 28621-1-AP (same clone as 28621-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KLHL32 antibody (28621-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (red). This data was developed using the same antibody clone with 28621-1-PBS in a different storage buffer formulation.

KLHL32 Polyclonal antibody, PBS Only

KLHL32 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28621-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BKLHD5, BTB and kelch domain-containing protein 5, Kelch like protein 32, Kelch-like protein 32, KIAA1900

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28621-1-PBS targets KLHL32 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29003

Product name: Recombinant human KLHL32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-318 aa of BC071627

Sequence: KHLSELLKSRPEEVLTLPYCLLQEVLKSDRLTSLSEEQIWQLAVRWLEHNCHYQYMDELLQYIRFGLMDVDTLHTVALSHPLVQASETATALVNEALEYHQSIYAQPVWQTRRTKPRFQSDTLYIIGGKKREVCKVKELRYFNPVDQENA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 32 (Drosophila)
别名 BKLHD5, BTB and kelch domain-containing protein 5, Kelch like protein 32, Kelch-like protein 32, KIAA1900
计算分子量 470 aa, 53 kDa
观测分子量70 kDa
GenBank蛋白编号BC071627
基因名称 KLHL32
Gene ID (NCBI) 114792
RRIDAB_3742296
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NJ5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLHL32 (Kelch-like protein 32), also known BKLHD5, which is located in the cytoplasm. The expression level is high in the central nervous system, especially in astrocytes. In addition, it is also expressed in the motor cilia of fallopian tube. But the overall expression abundance is low. KLHL32 is mainly involved in the intracellular ubiquitination process. Ubiquitin is an important cellular regulatory mechanism, through which protein can be labeled to degrade or change its function. KLHL32 interacts with other protein through its BTB domain and Kelch repeat domain to help regulate this process. It is also a prognostic marker in glioblastoma and renal clear cell carcinoma. The molecular weight of KLHL32 is 70 kDa.

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