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验证数据展示

  • WB analysis using 28621-1-AP

    WB analysis using 28621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28621-1-AP (KLHL32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 28621-1-AP

    IF Staining of U2OS using 28621-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KLHL32 antibody (28621-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (red).

KLHL32 Polyclonal antibody

KLHL32 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28621-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RP1 39B17.1, KIAA1900, Kelch-like protein 32, Kelch like protein 32, BTB and kelch domain-containing protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, PC-3 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28621-1-AP targets KLHL32 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KLHL32 fusion protein Ag29003 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 32 (Drosophila)
别名 RP1 39B17.1, KIAA1900, Kelch-like protein 32, Kelch like protein 32, BTB and kelch domain-containing protein 5
计算分子量 470 aa, 53 kDa
观测分子量70 kDa
GenBank蛋白编号BC071627
基因名称 KLHL32
Gene ID (NCBI) 114792
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL32 (Kelch-like protein 32), also known BKLHD5, which is located in the cytoplasm. The expression level is high in the central nervous system, especially in astrocytes. In addition, it is also expressed in the motor cilia of fallopian tube. But the overall expression abundance is low. KLHL32 is mainly involved in the intracellular ubiquitination process. Ubiquitin is an important cellular regulatory mechanism, through which protein can be labeled to degrade or change its function. KLHL32 interacts with other protein through its BTB domain and Kelch repeat domain to help regulate this process. It is also a prognostic marker in glioblastoma and renal clear cell carcinoma. The molecular weight of KLHL32 is 70 kDa.

实验方案

Product Specific Protocols
WB protocol for KLHL32 antibody 28621-1-APDownload protocol
IF protocol for KLHL32 antibody 28621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols