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验证数据展示

  • WB analysis of Jurkat using 25224-1-AP (same clone as 25224-1-PBS)

    WB analysis of Jurkat using 25224-1-AP (same clone as 25224-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25224-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 25224-1-AP (same clone as 25224-1-PBS)

    IHC staining of human liver using 25224-1-AP (same clone as 25224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25224-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 25224-1-AP (same clone as 25224-1-PBS)

    IHC staining of human liver using 25224-1-AP (same clone as 25224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25224-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 25224-1-AP (same clone as 25224-1-PBS)

    IHC staining of human liver using 25224-1-AP (same clone as 25224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25224-1-PBS in a different storage buffer formulation.

KLHL28 Polyclonal antibody, PBS Only

KLHL28 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25224-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

BTB/POZ domain-containing protein 5, BTBD5, Kelch-like protein 28

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

25224-1-PBS targets KLHL28 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18665

Product name: Recombinant human KLHL28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 222-571 aa of BC121009

Sequence: NSVRLPLLSVKFLTRLYEANHLIRDDRTCKHLLNEALKYHFMPEHRLSHQTVLMTRPRCAPKVLCAVGGKSGLFACLDSVEMYFPQNDSWIGLAPLNIPRYEFGICVLDQKVYVIGGIATNVRPGVTIRKHENSVECWNPDTNTWTSLERMNESRSTLGVVVLAGELYALGGYDGQSYLQSVEKYIPKIRKWQPVAPMTTTRSCFAAAVLDGMIYAIGGYGPAHMNSVERYDPSKDSWEMVASMADKRIHFGVGVMLGFIFVVGGHNGVSHLSSIERYDPHQNQWTVCRPMKEPRTGVGAAVIDNYLYVVGGHSGSSYLNTVQKYDPISDTWLDSAGMIYCRCNFGLTAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 28 (Drosophila)
别名 BTB/POZ domain-containing protein 5, BTBD5, Kelch-like protein 28
计算分子量 571 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC121009
基因名称 KLHL28
Gene ID (NCBI) 54813
RRIDAB_2879972
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXS3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLHL28 also named as BTBD5 or Kelch like protein 28 is a 571 amino acid protein, which contains one BTB domain and six kelch repeats. KLHL28 localizes in the cytoplasm and belongs to BTB/kelch-associated proteins family. KLHL28 may play a role in organizing the actin cytoskeleton of the brain cells. KLHL28 is expressed as two isoforms produced by alternative splicing.

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