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KLHL25 Polyclonal antibody

KLHL25 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 27482-1-AP

Print datasheet

Synonyms

ENC 2, ENC2, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

验证数据展示

  • WB analysis using 27482-1-AP

    WB analysis using 27482-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27482-1-AP (KLHL25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27482-1-AP

    IHC staining of mouse brain using 27482-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27482-1-AP (KLHL25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 27482-1-AP

    IF Staining of A549 using 27482-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using KLHL25 antibody (27482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27482-1-AP targets KLHL25 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen KLHL25 fusion protein Ag25765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 25 (Drosophila)
Synonyms ENC 2, ENC2, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25
Observed Molecular Weight66 kDa
GenBank Accession NumberBC028100
Gene Symbol KLHL25
Gene ID (NCBI) 64410
RRIDAB_2880883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0H3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL25 antibody 27482-1-APDownload protocol
IHC protocol for KLHL25 antibody 27482-1-APDownload protocol
IF protocol for KLHL25 antibody 27482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols