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  • Featured Product
  • KD/KO Validated

KLHL21 Polyclonal antibody

KLHL21 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16952-1-AP

Print datasheet

Synonyms

kelch like 21 (Drosophila), Kelch like protein 21, KIAA0469, KLHL21

验证数据展示

  • WB analysis of A431 using 16952-1-AP

    WB analysis of A431 using 16952-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16952-1-AP (KLHL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16952-1-AP

    IP experiment of HeLa using 16952-1-AP

    IP result of anti-KLHL21 (IP:16952-1-AP, 3ug; Detection:16952-1-AP 1:300) with HeLa cells lysate 1800ug.

  • IHC staining of human liver cancer using 16952-1-AP

    IHC staining of human liver cancer using 16952-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 16952-1-AP

    IHC staining of human brain using 16952-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16952-1-AP (KLHL21 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16952-1-AP targets KLHL21 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KLHL21 fusion protein Ag10582 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 21 (Drosophila)
Synonyms kelch like 21 (Drosophila), Kelch like protein 21, KIAA0469, KLHL21
Calculated Molecular Weight 597 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC034039
Gene Symbol KLHL21
Gene ID (NCBI) 9903
RRIDAB_2265294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJP4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL21 antibody 16952-1-APDownload protocol
IHC protocol for KLHL21 antibody 16952-1-APDownload protocol
IP protocol for KLHL21 antibody 16952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Breast Cancer Res Treat

Tumor-promoting mechanisms of macrophage-derived extracellular vesicles-enclosed microRNA-660 in breast cancer progression.

Authors - Changchun Li
  • KD Validated
View Article