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验证数据展示

  • WB analysis of HEK-293 using 18111-1-AP

    WB analysis of HEK-293 using 18111-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18111-1-AP (KLHL20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18111-1-AP

    IHC staining of human kidney using 18111-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 18111-1-AP

    IHC staining of human kidney using 18111-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18111-1-AP (KLHL20 antibody) at dilution of 1:50 (under 40x lens).

KLHL20 Polyclonal antibody

KLHL20 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18111-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

kelch like 20 (Drosophila), Kelch like protein 20, Kelch like protein X, KHLHX, KLEIP, KLHL20, KLHLX, RP3 383J4.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18111-1-AP targets KLHL20 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12567

Product name: Recombinant human KLHL20 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 261-609 aa of BC063418

Sequence: LLSPKFLVGTVGSDPLIKSDEECRDLVDEAKNYLLLPQERPLMQGPRTRPRKPIRCGEVLFAVGGWCSGDAISSVERYDPQTNEWRMVASMSKRRCGVGVSVLDDLLYAVGGHDGSSYLNSVERYDPKTNQWSSDVAPTSTCRTSVGVAVLGGFLYAVGGQDGVSCLNIVERYDPKENKWTRVASMSTRRLGVAVAVLGGFLYAVGGSDGTSPLNTVERYNPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVGGRDDTTELSSAERYNPRTNQWSPVVAMTSRRSGVGLAVVNGQLMAVGGFDGTTYLKTIEVFDPDANTWRLYGGMNYRRLGGGVGVIKMTHCESHIW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 20 (Drosophila)
别名 kelch like 20 (Drosophila), Kelch like protein 20, Kelch like protein X, KHLHX, KLEIP, KLHL20, KLHLX, RP3 383J4.3
计算分子量 609 aa, 68 kDa
观测分子量 65-67 kDa
GenBank蛋白编号BC063418
基因名称 KLHL20
Gene ID (NCBI) 27252
RRIDAB_2131189
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2M5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL20 antibody 18111-1-APDownload protocol
IHC protocol for KLHL20 antibody 18111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols