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KLHL15 Polyclonal antibody

KLHL15 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25066-1-AP

Print datasheet

Synonyms

kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15

验证数据展示

  • WB analysis of HeLa using 25066-1-AP

    WB analysis of HeLa using 25066-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25066-1-AP (KLHL15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25066-1-AP

    IHC staining of human testis using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 25066-1-AP

    IHC staining of human brain using 25066-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25066-1-AP (KLHL15 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25066-1-AP is KLHL15 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen KLHL15 fusion protein Ag16878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 15 (Drosophila)
Synonyms kelch like 15 (Drosophila), Kelch like protein 15, KIAA1677, KLHL15
Calculated Molecular Weight 604 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC109058
Gene Symbol KLHL15
Gene ID (NCBI) 80311
RRIDAB_2879880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL15 antibody 25066-1-APDownload protocol
IHC protocol for KLHL15 antibody 25066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Micropeptide PACMP inhibition elicits synthetic lethal effects by decreasing CtIP and poly(ADP-ribosyl)ation.

Authors - Chuanchao Zhang
View Article