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验证数据展示

  • WB analysis of HEK-293 using 51131-1-AP

    WB analysis of HEK-293 using 51131-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51131-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51131-1-AP

    IHC staining of human ovary tumor using 51131-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51131-1-AP (KLHL14 antibody) at dilution of 1:50 (under 10x lens).

KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51131-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51131-1-AP targets KLHL14 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 14 (Drosophila)
别名 kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14
计算分子量 44 kDa, 71 kDa
观测分子量 55 kDa, 70 kDa
GenBank蛋白编号BC021267
基因名称 KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131168
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2G3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL14 antibody 51131-1-APDownload protocol
IHC protocol for KLHL14 antibody 51131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols