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KLHDC5 Polyclonal antibody

KLHDC5 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24847-1-AP

Print datasheet

Synonyms

kelch domain containing 5, KIAA1340, KLHDC5, KLHL42

验证数据展示

  • WB analysis of HeLa using 24847-1-AP

    WB analysis of HeLa using 24847-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 24847-1-AP

    WB analysis of mouse brain using 24847-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24847-1-AP

    IHC staining of human kidney using 24847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 24847-1-AP

    IHC staining of human kidney using 24847-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 24847-1-AP

    IF Staining of HeLa using 24847-1-AP

    Immunofluorescent analysis of HeLa cells using 24847-1-AP (KLHDC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24847-1-AP is KLHDC5 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen KLHDC5 fusion protein Ag20448 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch domain containing 5
Synonyms kelch domain containing 5, KIAA1340, KLHDC5, KLHL42
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC137116
Gene Symbol KLHDC5
Gene ID (NCBI) 57542
RRIDAB_2879754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2K6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHDC5 also named as kelch domain containing 5 or KLHL42 is a 505 amino acid protein, which contains one BTB domain and six Kelch repeats. KLHDC5 localizes in cytoplasm and may involve in microtubule dynamics throughout mitosis.

实验方案

Product Specific Protocols
WB protocol for KLHDC5 antibody 24847-1-APDownload protocol
IHC protocol for KLHDC5 antibody 24847-1-APDownload protocol
IF protocol for KLHDC5 antibody 24847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Acta Pharmacol Sin

Satellite cell-derived exosome-mediated delivery of microRNA-23a/27a/26a cluster ameliorates the renal tubulointerstitial fibrosis in mouse diabetic nephropathy

Authors - Jia-Ling Ji
View Article